FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759243
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTCTGAGGGAGA30590.4029013109109995No Hit
GTATATTACTGTGCGAGACATTTGTCTCCACATTACTATGATTCGGGGAC20720.2729034051021873No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19610.25828357982885586No Hit
GTATACTAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17650.23246839285973003No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14680.19335048199324853No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14650.1929553515804558No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT14240.18755523593895498No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13540.17833552630712432No Hit
GTTAGTATACTGCTGACAATAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACT13510.17794039589433158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12940.1704329180512695No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12870.16951094708808642No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12310.1621351793826219No Hit
GTCTCTAGTGGTTCCATCACCAGTAGTAATTACTACTGGGGCTGGATCCG12190.16055465773145094No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12010.1581838752546945No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGC11870.15633993332832835No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11540.15199349878760818No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11410.15028126699883965No Hit
CTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT11160.1469885135589001No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10920.14382747025655818No Hit
CACCTGCACTGTCTCTAGTGGTTCCATCACCAGTAGTAATTACTACTGGG10870.14316891956857028No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10800.1422469486053872No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT10590.139481035715838No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10320.13592486200070333No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10180.13408092007433722No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10180.13408092007433722No Hit
GTATACTGCTGACAATAATAAACTGCAAAATCTTCAGACTGCAGGCTGGT10170.13394920993673962No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10050.13236868828556864No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAG10030.13210526801037348No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTA9810.12920764498322673No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTATTGTCAGCAGTATACTAACTGG9770.1286808044328364No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA9610.12657344223127512No Hit
GCAGTATACTAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGG9590.12631002195607993No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9550.12578318140568961No Hit
GAGTATTGGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATC9390.12367581920412832No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9320.12275384824094525No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9120.12011964548899363No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8890.11709031232424928No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8820.11616834136106621No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8780.1156415008106759No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8760.11537808053548075No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8490.11182190682034607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8470.11155848654515091No Hit
GGATACCAGTGGCCCTGGCGGATGCACCATGGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG8420.11089993585716298No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8390.11050480544437026No Hit
GTCTGAGGGAGAACTGGTTCTTGGACGTGTCAACGGATACGGTGAGTCGA8350.10997796489397992No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTTAGTATACTG8230.10839744324280895No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA8230.10839744324280895No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7970.10497297966527185No Hit
GGCCAAAAGTGTACGGAGGCCAGTTAGTATACTGCTGACAATAATAAACT7810.10286561746371058No Hit
CTATAATAGACACTCCCCATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAATTACTA7790.10260219718851539No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7700.10141680595013716No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7660.10088996539974686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5400.044.601441
GTGGTAT1700.044.5264781
GTATCAA14150.042.066291
AACGGAT8550.041.93731370
CTACGCT856.730261E-1140.49728828
CGGTGTT350.001357621239.36876332
CGTATGC655.347265E-737.242441
CTCGTAT753.985042E-836.8340639
TATCAAC16150.036.4307562
ATCAACG16750.034.920363
CAACGCA16850.034.9150125
TCAACGC16800.034.8141374
TCGTATG806.978371E-834.56163840
AACGCAG16950.034.50156
AACTATT400.002616865634.44312731
TACGCTG1003.8926373E-1034.42950429
TATTAGA501.8906317E-434.4068222
ACTACGC1154.9112714E-1132.9238927
TTATACA1056.6211214E-1032.7684022
GAGACTA1056.6211214E-1032.76840224
Produced by FastQC (version 0.11.5)