FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759243
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTCTGAGGGAGA30170.39736948513190107No Hit
GTATATTACTGTGCGAGACATTTGTCTCCACATTACTATGATTCGGGGAC19260.25367372501294055No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19140.25209320336176955No Hit
GTATACTAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17810.23457575506129133No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16000.21073622015612922No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT14130.18610642442538158No Hit
GTTAGTATACTGCTGACAATAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACT13570.17873065671991706No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13310.17530619314238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12370.1629254402082074No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGC12120.15963268676826786No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11920.15699848401631625No Hit
GTCTCTAGTGGTTCCATCACCAGTAGTAATTACTACTGGGGCTGGATCCG11870.15633993332832835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11830.15581309277793803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11680.15383744071397432No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11640.153310600163584No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10740.14145668777980172No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10700.1409298472294114No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10560.13908590530304527No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10390.1368468329638864No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10170.13394920993673962No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT10130.1334223693863493No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10100.13302723897355656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10020.13197355787277593No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9930.13078816663439768No Hit
GTATACTGCTGACAATAATAAACTGCAAAATCTTCAGACTGCAGGCTGGT9830.1294710652584219No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTATTGTCAGCAGTATACTAACTGG9780.12881251457043397No Hit
CTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT9770.1286808044328364No Hit
GAGTATTGGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATC9560.1259148915432872No Hit
CACCTGCACTGTCTCTAGTGGTTCCATCACCAGTAGTAATTACTACTGGG9550.12578318140568961No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAGCAACTTAGCCTGGTA9530.12551976113049446No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTGGCAG9450.12446608002971381No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA9320.12275384824094525No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9240.12170016714016461No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9050.11919767452581058No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8940.11774886301223718No Hit
GCAGTATACTAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGG8940.11774886301223718No Hit
GTCTGAGGGAGAACTGGTTCTTGGACGTGTCAACGGATACGGTGAGTCGA8920.11748544273704202No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8610.11340242847151702No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTTAGTATACTG8490.11182190682034607No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8330.10971454461878476No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8310.10945112434358961No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8000.10536811007806461No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT7980.10510468980286942No Hit
GGCCAAAAGTGTACGGAGGCCAGTTAGTATACTGCTGACAATAATAAACT7950.1047095593900767No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7890.1039192985644912No Hit
CTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAAGAATCGACTCACCGTATCCGTTGACACGTCCAAGAA7880.10378758842689362No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7800.10273390732611297No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7780.10247048705091782No Hit
GGATACCAGTGGCCCTGGCGGATGCACCATGGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG7750.10207535663812509No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7740.10194364650052751No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7740.10194364650052751No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT7640.1006265451245517No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7600.10009970457416137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)