FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086344
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATAAGTATCACTACCTGCATATGAG26280.24191232243193686No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATAAGTATCA24340.22405425905606327No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22260.2049074694571885No Hit
GTACCAACACCACCCAGGCGAAGCCCCCAAACTCCTTCTTTATGAGGTCA20300.1868653023351719No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19920.1833673311584544No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT19650.18088193058552357No Hit
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA18260.1680867202285832No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18190.1674423571170826No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17290.15915768854064644No Hit
GTAATAATCAGCCTCGTCCTCAGCCAGGAGCCCAGAGATTGTCAGGGAGG16940.15593587298314346No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15430.14203604014934496No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15150.1394585877033426No Hit
GTGTTGGTACCAGGAGACAACGTTATAACCCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA14710.135408305288196No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14690.13522420154205297No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14510.13356726782676573No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14220.13089776350769186No Hit
GTTTATTACTGTGCGAGAGCGTACAAAGTTCTTGGTACTAGGGAAACTGA14160.13034545226926278No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGGGTTATAACGTTGTCTCCTGGTACCAACACCACCCAGGCGAAGC14060.12942493353854764No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13790.12693953296561677No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13760.12666337734640226No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13490.12417797677347138No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13350.1228892505504702No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGGGTTATAACGTTGTCTCCTGGTACCAACACCA13010.11975948686603875No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTGATACTTATGTGTTATTCGG12950.11920717562760967No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12940.11911512375453814No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG12780.11764229378539394No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12730.11718203442003639No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12440.11451253010096249No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12300.1132238038779613No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12110.11147481828960257No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12080.11119866267038801No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12060.11101455892424499No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11930.10981788457431532No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11830.10889736584360019No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11300.10401861657080998No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11150.10263783847473727No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11070.10190142349016516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT200.006902124352.5954960
CGCTAGA200.007230227351.9814244
ATACTAA400.00888401949.33891770
GTGGTAT2200.043.8232271
GTATCAA17650.043.6990851
TATCAAC19650.039.422942
GGTATCA6650.039.351471
TGGTATC2600.037.077782
ATCAACG20950.036.9766463
CAACGCA21250.036.2926065
AACGCAG21500.035.8689466
TCAACGC21600.035.7045334
AGAGTAC23250.033.02105711
CGCAGAG23850.032.334698
CAGAGTA23900.032.26704410
TGTATAA1800.030.60388214
ACGCAGA25450.030.301867
AGTACGG22100.030.22167613
GCAGAGT26000.029.5284429
GAGTACG22750.029.35819612
Produced by FastQC (version 0.11.5)