FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086344
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATAAGTATCACTACCTGCATATGAG22970.21144315244526596No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22670.20868159625312055No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATAAGTATCA20670.1902712216388179No Hit
GTACCAACACCACCCAGGCGAAGCCCCCAAACTCCTTCTTTATGAGGTCA18990.1748065069628037No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17440.16053846663671914No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT17330.15952589603293246No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16960.15611997672928649No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16910.15565971736392892No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16530.15216174618721143No Hit
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA16280.1498604493604236No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16060.1478353081528503No Hit
GTAATAATCAGCCTCGTCCTCAGCCAGGAGCCCAGAGATTGTCAGGGAGG15420.14194398827627344No Hit
GTGTTGGTACCAGGAGACAACGTTATAACCCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA14660.13494804592283843No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTGATACTTATGTGTTATTCGG14310.13172623036533546No Hit
GTTTATTACTGTGCGAGAGCGTACAAAGTTCTTGGTACTAGGGAAACTGA14300.13163417849226397No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGGGTTATAACGTTGTCTCCTGGTACCAACACCACCCAGGCGAAGC14230.13098981538076337No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14090.12970108915776218No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13950.128412362934761No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13780.1268474810925453No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13760.12666337734640226No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13630.12546670299647256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12980.1194833312468242No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGGGTTATAACGTTGTCTCCTGGTACCAACACCA12790.11773434565846547No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12390.11405227073560494No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG12350.11368406324331888No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12340.11359201137024735No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11770.10834505460517112No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11690.10760863962059901No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11540.1062278615245263No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11290.10392656469773848No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11270.10374246095159545No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11080.10199347536323669No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11040.10162526787095064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATA200.00730719251.842440
GTGGTAT2500.045.440451
GTATCAA18500.043.356371
TGGTATC2750.041.258152
TATCAAC19650.040.4182852
CCCTATA350.001370717339.2934762
ATCAACG20750.038.2703483
TCAACGC20800.038.0130774
CAACGCA21200.036.9698335
GGTATCA6850.036.686051
AACGCAG21700.036.434826
AGAGTAC22850.034.75795711
CAGAGTA23250.033.85642210
CGCAGAG23550.033.425138
GCAGAGT24200.032.669399
CAAGGGA8050.032.2519470
ACGCAGA25350.031.3243837
AGTACGG23950.031.29532413
GAGTACG24350.031.06363112
GTACGGG27250.027.50543414
Produced by FastQC (version 0.11.5)