FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504977
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45200.040.236671
GGTATCA19700.039.8377571
CTCGTAT2300.035.98694639
TATCAAC51450.035.2150232
TCAACGC53100.034.1816254
ATCAACG53650.033.705143
CAACGCA54550.033.2730375
AACGCAG55900.032.4673236
TCGTATG2700.031.9736740
GACCGAT3150.031.4326226
ACGCAGA59600.030.451747
CGCAGAG61300.029.7185428
AGAGTAC61150.029.62407311
TCTCGTA2850.029.00118638
CAGAGTA63250.028.74837910
GCAGAGT65150.028.1194349
ACCGATC3550.027.90019427
AGTACGG51250.027.89117613
GAGTACG51950.027.58102812
TGGTATC7950.027.0391032
Produced by FastQC (version 0.11.5)