FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504977
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26370.17521862460356535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45400.039.9965251
GGTATCA19000.038.0845451
TATCAAC49950.035.967232
TCAACGC51600.034.4047054
ATCAACG52200.034.013773
CAACGCA52300.033.9442185
AACGCAG53400.033.3076976
AGAGTAC57600.031.41349611
ACGCAGA56750.031.4015567
CGCAGAG58250.030.8258888
CAGAGTA59200.030.50387810
GCAGAGT59650.030.0452799
GTGGTAT8100.029.9184991
AGTACGG53350.027.6565313
GAGTACG53900.027.3752312
TACGGGG24250.026.84075415
TGGTATC9000.025.757182
GTACGGG59050.024.75524314
TCGCGTG750.001360380124.56013567
ACGGGGC6050.024.22389216
Produced by FastQC (version 0.11.5)