FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651706
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60900.36870968562201745No Hit
CATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA55000.33298904284418657No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC37760.2286121137781179No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37270.22564548412368785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36550.22128635483554582No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35680.2160190736123741No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35010.21196266163590857No Hit
GACTTACAGTTTCCCGCTCGCTTTCGGCGGAGGGACCATGGTGGAGATCAAAGGAACTGTGGCTGCACCAT33130.2005804907168709No Hit
CTGTAAGTCTGCTGACAATAGTAAGTCGCGAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC33010.19985396916884723No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32440.19640299181573473No Hit
GCATAGATGAGGCGCCTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGAC32100.19434451409633435No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29860.18078277853322564No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCG28860.17472843229969498No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28370.17176180264526494No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28020.16964278146352924No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAA27520.1666156083467639No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27120.16419386985335163No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26240.15886604516784464No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT26220.15874495824317403No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26140.15826061054449156No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25910.1568681109107795No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25840.15644430667443238No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25690.15553615473940277No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25430.1539620247186848No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT25300.15317495970832581No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTC25110.152024633923955No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24360.147483874248807No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24320.14724170039946577No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24110.14597028769042433No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22720.1375547464258167No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22420.1357384425557575No Hit
CCCCTAGGCGCCTCATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA22370.13543572524408096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22030.13337724752468055No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT21850.13228746520264503No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21750.13168203057929195No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21610.13083442210659765No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC20810.12599094511977313No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20720.12544605395875535No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20720.12544605395875535No Hit
GTAGGTGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATGTTAGCAGATGGGTAGTCTGGTATCAGCAGAAA20320.1230243154653431No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19900.12048149004726023No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19410.11751486039283021No Hit
CCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAACTGTAAGTCTGCTGA19150.11594073037211224No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18670.11303464418001752No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18630.11279247033067628No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTCCCGCTCGCTTT18460.11176323147097608No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT18280.11067344914894055No Hit
GGATGTTAGCAGATGGGTAGTCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAGGCGCCTCATCTATGCTGCATC18130.10976529721391094No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18120.10970475375157564No Hit
GTCTCCATCTTTCGTGTCTGCATCTGTAGGTGACAGAGTCACCATCACTT18090.10952312336456974No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18040.1092204060528932No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17720.10728301525816339No Hit
GCCTTAGAGTCGAGGACACGGCCGTATATTATTGTGCCAGCCTCTATGAC17490.10589051562445131No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16650.10080486478828557No Hit
GTCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAGGCGCCTCATCTATGCTGCATCCAGTTTGCAAAGTGGGGTC16560.10025997362726781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC200.00756035451.3989264
GTATCAA35850.040.9708861
GTGGTAT4450.040.1018181
CTAACGC350.001344970439.4465438
TATCAAC39100.037.524412
TCAACGC41000.035.9374584
ATCAACG40950.035.898753
CAACGCA41550.035.3803565
AACGCAG42200.034.8353966
CGCAGAG44600.033.036688
AGAGTAC44650.032.84918211
CAGAGTA45500.032.30691510
GCAGAGT45800.032.0204859
CCGCGTA450.00478050630.4576999
CCTTTGT13400.029.77973670
GGGTTAC703.9936393E-529.4153441
ACGCAGA52050.028.3089437
AGTACGG45000.028.10148213
GAGTACG45700.027.67104312
ACGGGTC2150.027.10796216
Produced by FastQC (version 0.11.5)