FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536120
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46770.3044684009061792No Hit
CATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA41930.2729604457985053No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33360.21717053355206623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33320.21691013722886232No Hit
GCATAGATGAGGCGCCTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGAC32320.2104002291487644No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC28790.1874202536260188No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28580.18605317292919824No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT27830.1811707418691248No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27360.1781110850714788No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27300.1777204905866729No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27090.17635340988985235No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26680.1736843475770122No Hit
CTGTAAGTCTGCTGACAATAGTAAGTCGCGAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC26680.1736843475770122No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26590.17309845584980338No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCG25790.16789052938572507No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT25460.16574225971929277No Hit
GACTTACAGTTTCCCGCTCGCTTTCGGCGGAGGGACCATGGTGGAGATCAAAGGAACTGTGGCTGCACCAT24650.16046923417441344No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24540.1597531442856027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24440.1591021534775929No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22730.14797021066062546No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22720.1479051115798245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22420.14595213915579514No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22140.14412936489336772No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21980.14308777960055205No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAA21810.1419810952269354No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT20910.13612217795484727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20400.13280212483399734No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20320.1322813321875895No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC19980.1300679634403562No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA19490.12687810848110825No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19450.12661771215790432No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTC19310.12570632502669063No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19190.12492513605707888No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19010.12375335260266125No Hit
GCCTTAGAGTCGAGGACACGGCCGTATATTATTGTGCCAGCCTCTATGAC18790.12232117282503971No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18680.12160508293622893No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18260.11887092154258781No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17710.11529047209853398No Hit
GTAGGTGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATGTTAGCAGATGGGTAGTCTGGTATCAGCAGAAA17690.11516027393693201No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17660.11496497669452907No Hit
CCCCTAGGCGCCTCATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA17210.11203551805848502No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC17040.11092883368486836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16960.11040804103846054No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC16960.11040804103846054No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT16610.10812957321042628No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16530.10760878056401844No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTCTTC16030.10435382652396949No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG15830.10305184490794991No Hit
CCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAACTGTAAGTCTGCTGA15670.10201025961513424No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15670.10201025961513424No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15650.10188006145353229No Hit
GTCCCTGGAGACAGTGAATCGGCCCTGCACAGATTCTGCGTAATAAATGG15640.1018149623727313No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15570.10135926880712445No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA15450.1005780798375127No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTCCCGCTCGCTTT15370.10005728719110488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3750.051.5527421
GTATCAA27800.036.96631
CCTTTGT12000.035.03911270
ACGGGAT1700.031.90144316
TATCAAC32700.031.1971952
GTACGGA553.6002792E-430.8329771
ATCAACG33450.030.4818233
CAACGCA33750.030.3171635
TCAACGC33900.030.0820924
AACGCAG34200.030.0173196
CGCAGAG35800.028.8641018
AGAGTAC36050.028.4815411
TCGCTAT1551.8189894E-1228.46910924
CAGAGTA36650.028.1013210
GCAGAGT37800.027.247279
AGTATAC851.6344298E-423.9112833
AGTACGG38450.023.88355813
GAGTACG38900.023.78149412
ACGCAGA44650.023.067127
CTCGCTA1804.2200554E-1022.62412523
Produced by FastQC (version 0.11.5)