FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219048
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22980.18850775359132702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34150.037.7121621
GGTATCA15750.036.5353281
TCAACGC38450.033.285084
GTGGTAT6900.033.259061
TATCAAC38950.032.951062
ATCAACG38950.032.8551033
CAACGCA38950.032.769945
AACGCAG41100.030.972446
ACGCAGA42000.030.3087467
CGCAGAG42950.029.6383538
AGAGTAC43100.029.30062311
CAGAGTA44350.028.70275710
TGGTATC7550.028.5587482
GCAGAGT47650.026.5713219
AGTACGG38550.026.36802713
GAGTACG39050.026.0304112
GTACGGG43250.023.34433614
TACGGGG17000.021.9443715
TATCGAC800.002913574721.54453735
TACGGGT6400.021.39072815
Produced by FastQC (version 0.11.5)