FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425660
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCG40710.2855519548840537No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39160.27467979742715654No Hit
GTATAGGGGCCTGGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT38470.26983993378505394No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC36400.2553203428587461No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGCCACTGCTGTCTG36080.2530757684160319No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35290.24753447526058106No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34590.24262446866714366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG34320.2407306089811035No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33230.2330850272856081No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTG32580.22852573544884475No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31740.22263372753671984No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC30080.21098999761513965No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGG29680.20818427956174687No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT29050.20376527362765315No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28800.20201169984428263No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28620.20074912672025588No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC28580.20046855491491658No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28240.19808369456953268No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28020.19654054964016668No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCAC27960.19611969193215772No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27780.19485711880813097No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC27390.19212154370607298No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27190.19071868467937658No Hit
GGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTC27110.190157541068698No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26770.1877726807233141No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26690.18721153711263555No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26520.1860191069399436No Hit
TCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCA26360.18489681971858649No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25920.18181052985985438No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25920.18181052985985438No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25530.1790749547577964No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATG25250.1771109521204214No Hit
GGCCAAGCCAGGCCCCTATACTATTAATATATGAGGACACTGAGAGGCCC24750.1736038045536804No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24570.17234123142965363No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24540.17213080257564917No Hit
GGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT23990.16827294025223405No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA23940.16792222549555996No Hit
CCCCTATACTATTAATATATGAGGACACTGAGAGGCCCTCAGGGATCCCT23520.1649762215394975No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22430.15733063984400208No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22420.15726049689266727No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22420.15726049689266727No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC22170.15550692310929676No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21450.1504566306131897No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21170.14849262797581472No Hit
GACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCGGCGGAGGGACCAAGCTGACCGTC21080.14786134141380133No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20770.1456869099224219No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20270.1421797623556809No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGT20080.1408470462803193No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19750.1385323288862702No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19310.13544603902753813No Hit
GTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGA19080.13383275114683724No Hit
CCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGACTGTTGTCCC18680.13102703309344443No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18600.13046588948276588No Hit
CCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG18380.12892274455339983No Hit
CTATTAATATATGAGGACACTGAGAGGCCCTCAGGGATCCCTGAGCGATT18220.1278004573320427No Hit
GCAATATGCTTATTGGTACCAGCAGAGGCCAAGCCAGGCCCCTATACTATTAATATATGAGGACACTGAGAGGCC18200.12766017142937305No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCG17960.12597674059733738No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17540.12303073664127492No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC17500.12275016483593565No Hit
CTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCCGGGGA17370.12183830646858299No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG17340.12162787761457851No Hit
CTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGG16700.11713872872915002No Hit
GCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTG16660.11685815692381073No Hit
CAGTCACGTTGACCATCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTAT16630.11664772806980626No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16270.11412258182175274No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16270.11412258182175274No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16200.113631581162409No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16190.11356143821107417No Hit
GCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGCCACTGCTGTCTGCTGACTGACAGTAATAGTCACCCTCGT16130.11314058050306525No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15900.11152729262236438No Hit
GCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTACCAGCAGAGG15770.11061543425501172No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15740.11040500540100726No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15640.10970357588765905No Hit
GGGACAACAGTCACGTTGACCATCAGTGGAGTCCGGGCAGAGGACGAGGG15610.10949314703365459No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGC15460.10844100276363228No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGT15120.10605614241824839No Hit
GCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG15070.10570542766157429No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15050.10556514175890466No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAG15050.10556514175890466No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15040.10549499880756982No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT14960.10493385519689126No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14890.10444285453754752No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14800.10381156797553415No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14770.10360113912152968No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAAGCAATATGCTTATTGGTAC14700.10311013846218593No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14360.10072527811680204No Hit
GAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGAC14330.10051484926279758No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14320.10044470631146275No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14300.10030442040879313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG200.007849350.9132164
GTGGTAT3600.044.322691
GGTATCA12750.042.86931
GTATCAA33500.041.4485361
AGGGGGG4900.040.61036370
TGGTATC3900.040.0371362
TATACAC2900.038.6224673
CTTATAC3100.038.3298451
TATCAAC36350.038.1000482
TCGTATG2800.036.90110440
CTCGTAT2550.036.39185339
TCTCGTA2600.035.63885538
ATCAACG38850.035.5571943
TCAACGC39050.035.463244
CAACGCA39600.035.056415
TTATACA3100.035.0395132
TCGACGT3050.034.56403729
AACGCAG40500.034.2773786
CGTATGC3050.033.94528241
ACTCGAC3150.033.44443527
Produced by FastQC (version 0.11.5)