FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592522
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26180.1643933333417058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42450.037.9290351
GGTATCA17900.037.7824251
TCAACGC46700.034.1263164
ATCAACG46850.034.0902063
CAACGCA47750.033.376945
TATCAAC48150.033.2556042
AACGCAG48700.033.0073626
GTGGTAT7550.031.829351
ACGCAGA51300.031.2008537
TGGTATC7850.030.5754342
CGCAGAG53050.030.1716088
AGAGTAC54200.029.21800411
CAGAGTA55550.028.5669510
GCAGAGT56950.027.9850569
AGTACGG47400.027.19392613
GAGTACG48500.026.29442212
GTACGGG53000.023.9325114
AATAGCG2850.023.5588762
TACGGGG20650.022.74808115
TACGGGA19600.021.51757215
Produced by FastQC (version 0.11.5)