FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030595
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC40120.19757755731694407No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39110.19260364572945368No Hit
GTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTACTGA38780.19097850629987762No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT38260.1884176805320608No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCAC38100.18762973414196332No Hit
GTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGTGG37930.18679254110248475No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT35600.17531807179669012No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCACGTCACTGAGG35160.17315121922392207No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34520.16999943366353212No Hit
CAGTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTA32660.16083955687864887No Hit
GAGTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGG31750.15635811178496942No Hit
GTGTCTGGTGGGTCCTTCAGTAGTTATTATTGGAGCTGGATCCGCCAGTA31460.15492995895291775No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG30650.15094098035304923No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30640.15089173370366812No Hit
GGACAGAGGGTCGCCATCTCCTGCTCTGGAAGCAGATCCAACATTGGGAA30600.15069474710614378No Hit
ACATATTACTGTGCGCGGGGTCAATCGGCTCACACCACTGACTGCACTACCACAACCTGCTTTAGTCAGGGACGG30070.14808467468894584No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29530.14542535562236683No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29030.1429630231533122No Hit
GTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCG28510.1404021973854954No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28280.13926952444973026No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27990.13784137161767857No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26980.1328674600301882No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26870.13232574688699617No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG26710.1315378004968987No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26430.13015889431422809No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26290.1294694412228928No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26030.12818902833898438No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA24620.12124525077625031No Hit
CAGTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGT24340.11986634459357971No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC24140.11888141160595787No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT23970.11804421856647929No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23290.11469544640856498No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23160.11405523996661077No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22980.11316880027775111No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22850.1125285938357969No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22680.11169140079631831No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22310.10986927476921789No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22030.10849036858654729No Hit
ACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22020.10844112193716621No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG21380.10528933637677627No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21240.10459988328544095No Hit
CTCCTGCTCTGGAAGCAGATCCAACATTGGGAACAATTTTGTATCTTGGTACCAGCAGTTTCCAGGAACAGCCCC21160.10420591009039223No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21150.10415666344101113No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCGCCATCTCCTGCTCTGG21070.1037626902459624No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20880.10282700390772162No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20840.10263001731019726No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20690.10189131756948087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)