FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030595
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41000.2019112624624802No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40610.1999906431366176No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36540.17994725683851284No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC35930.1769432112262662No Hit
GTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTACTGA35350.1740869055621628No Hit
GTGTCTGGTGGGTCCTTCAGTAGTTATTATTGGAGCTGGATCCGCCAGTA32730.16118428342431654No Hit
GTATTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCGC32120.15818023781206986No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31850.15685057827878035No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCAC31710.15616112518744507No Hit
CAGTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTA31660.1559148919405396No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29660.14606556206432106No Hit
GAGTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGG29300.1442926826866017No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG29140.14350473629650423No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27750.13665945203253235No Hit
GGACAGAGGGTCGCCATCTCCTGCTCTGGAAGCAGATCCAACATTGGGAA27740.13661020538315125No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCACGTCACTGAGG27440.13513280590171844No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG27200.13395088631657223No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26800.13198102034132853No Hit
GTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCG26690.1314393071981365No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT26670.13134081389937433No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26320.1296171811710361No Hit
ACATATTACTGTGCGCGGGGTCAATCGGCTCACACCACTGACTGCACTACCACAACCTGCTTTAGTCAGGGACGG26290.1294694412228928No Hit
GTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGTGG25470.1254312159736432No Hit
GTCCTGGGCCCAGTCTGTATTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCGCCATCTCCTG25280.12449552963540243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25280.12449552963540243No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24900.1226241569589209No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24860.12242717036139654No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24550.12090052423058266No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24270.11952161804791206No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA24170.11902915155410114No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23870.11755175207266837No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23650.11646832578628431No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC23630.11636983248752213No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23340.11494167965547045No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21910.10789940879397418No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21890.107800915495212No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21880.1077516688458309No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT21550.10612652941625483No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20900.10292549720648382No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20480.10085713793247793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5550.045.179931
TGGTATC5800.043.783722
GTATCAA44700.043.416681
GGTATCA16950.040.327291
TATCAAC49900.038.4432532
TCAACGC53350.035.943954
ATCAACG53550.035.6815833
CAACGCA54650.035.155165
AACGCAG55800.034.492126
CGCAGAG58150.033.155568
AGAGTAC59050.032.65424711
CAGAGTA60200.032.02729810
GCAGAGT60550.031.9546999
ACGCAGA66250.029.1543257
AGTACGG60150.028.69307113
GAGTACG61150.028.22384512
GACAGGT5300.026.5719621
TACGGGG29800.026.13820315
GTACGGG68800.025.03571314
GGGCAGT9250.024.1372221
Produced by FastQC (version 0.11.5)