FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150026
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA43050.3743393627622332No Hit
GTGTACTACTGTGCGAGAGGCCGCGTCCGATGGCCCCCCTTTGACTACTGGGGCCAGGGAAGCCTGGTCACCGT25450.22129934453655828No Hit
GTCTACAGTGGGTCCTTCAGAGGTTACTACTGCAACTGGATCCGCCAGCC23120.20103893303281842No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19870.17277870239455456No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19280.16764838360176204No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17800.15477910934187575No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17090.14860533587936273No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16950.14738797209802212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16650.14477933542372085No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC16140.14034465307740868No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16050.13956206207511831No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15680.13634474351014672No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15190.13208397027545465No Hit
AGTCAGGACATTAGCCATTATTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATT15060.1309535610499241No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15030.13069269738249398No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14270.12408415114093072No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13990.12164942357824954No Hit
CAGTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGA13970.12147551446662945No Hit
GAATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC12650.10999751309970383No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12570.1093018766532235No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT12470.10843233109512306No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12390.10773669464864272No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12380.10764974009283268No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12210.10617151264406197No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11920.10364983052557072No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11830.10286723952328035No Hit
GATCTATTCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCT11700.1017368302977498No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG11680.10156292118612971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG608.1227877E-443.69973870
TATGGAT14800.042.0757670
GTATCAA21300.041.252881
TATCAAC23900.036.4758342
CTACGCT1051.8189894E-1135.8509728
ATCAACG25400.034.5879333
GTGGTAT2500.034.190131
TCAACGC25800.034.051684
CAACGCA26150.033.5959245
ACTACGC1155.2750693E-1132.73206727
AACGCAG27050.032.478136
CTTATAC1307.2759576E-1231.560121
AGAGTAC28400.031.17501311
CAGAGTA28900.030.75393510
CGCAGAG29050.030.3597878
GCAGAGT29850.029.7751669
TATACAC1650.029.004763
TTCGGGG10450.028.22710270
ACGAGAC1353.4015102E-1027.86104622
TATAAGA1653.6379788E-1226.93416416
Produced by FastQC (version 0.11.5)