FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827841
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9400.040.1500471
GTATCAA21800.038.087291
TCGTATG655.2625546E-737.3190640
ATCAACG25400.033.0883333
ACGAGAC1350.033.04129422
TCAACGC25450.033.021334
CGTATGC958.3327905E-932.86364441
CAACGCA25700.032.8335845
TATCAAC26000.032.592572
CTCGTAT751.6255271E-632.30769739
AACGCAG26950.031.1815286
TCTCGTA1001.4149919E-831.12539338
TACCGAT450.004755496530.4886057
ACGCAGA28400.029.5895127
ACGGGTA1551.8189894E-1228.76920916
CGCAGAG29700.028.294358
AGAGTAC30250.027.89329311
CAGAGTA31250.027.00070810
AGTACGG24700.026.662113
GAGTACG24850.026.36313412
Produced by FastQC (version 0.11.5)