FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796294
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9030.1134003270148965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24000.039.766121
GGTATCA10450.036.3351481
TATCAAC27750.033.8672942
CAACGCA28150.033.258385
ATCAACG28150.033.258383
TCAACGC28650.032.5586934
AACGCAG29400.031.9605566
ACGCAGA30400.030.7968247
AGAGTAC31100.030.3271911
GTGGTAT4150.029.6739031
CGCAGAG31600.029.6273258
CAGAGTA32300.029.40838810
GCAGAGT32150.029.120489
GAGTACG25150.028.94359212
AGTACGG25300.028.6369113
TACGGGT5800.026.51522615
TGGTATC4900.026.50322
GTACGGG27050.026.15253814
ACGGGTA1608.731149E-1125.63460416
TACGGGA9550.024.69201515
Produced by FastQC (version 0.11.5)