FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334513
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7490.22390759103532598No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6840.20447635816844187No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6290.18803454574261688No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6290.18803454574261688No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6110.1826535889487105No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6090.1820557048604987No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG5860.17518003784606279No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5560.16621177652288552No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5280.15784139928792007No Hit
CTCCTGTACAGCCTCTGGGTTCACCGTCAGTACCAGCTACATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGG5090.15216150044990778No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5050.15096573227348414No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG4580.1369154562005064No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT4530.13542074597997686No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT4410.13183344145070594No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4340.12974084714196457No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC4240.1267514267009055No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA4130.12346306421574049No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC4060.12137046990699912No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG4050.12107152786289323No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA4030.1204736437746814No Hit
CTGCTATACGTCACCCCTCTCGAACAGTAATAAACGGCCGTGTCCTCGGC4010.11987575968646959No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGAAACA3940.11778316537772822No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC3870.11569057106898685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3820.1141958608484573No Hit
GTATGTGGCACCACCACTATAAATAAGTGAGACCCACTCCAGCCCCTTCC3720.11120644040739823No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG3680.11001067223097459No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3670.10971173018686867No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG3600.10761913587812731No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3580.10702125178991548No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3480.10403183134885641No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC3470.10373288930475048No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT3440.10283606317243277No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT3430.10253712112832684No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3380.1010424109077973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG150.002162144870.495460
GTGGTAT750.064.0941
TGGTATC900.053.4036872
GTACCAC200.00749872951.496416
GGTATCA3000.043.492361
GTATCAA6750.038.6598781
TTATACA501.913035E-434.3309442
TCTACAC553.347166E-431.2099483
TATCAAC8400.031.0613292
CAACGCA8400.031.0613295
ATCAACG8500.030.69593
TCAACGC8550.030.5163944
ACAACAC450.00473036330.5163923
ACCTAGG450.00473036330.5163922
AACGCAG8700.030.3848576
GACAGGA802.858087E-630.0440641
CAGCGCT2650.029.7966674
TGCTTTA2650.029.79666714
GCTTTAT2800.028.20463215
AGCGCTG2800.028.2004175
Produced by FastQC (version 0.11.5)