FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919121
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9840.10705880944946312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG300.003214496763.78949470
TAACGGT200.007526088551.4561926
TATACCG352.3503351E-549.005896
ATACCGC306.492092E-445.7363437
GGTATCA11300.042.5330921
GTATCAA28650.039.422911
ATCAACG32500.034.6132473
TCAACGC32550.034.351214
CAACGCA32650.034.0414625
TATCAAC33400.033.8896062
AACGCAG33250.033.324016
ACGCAGA35050.031.806667
AGAGTAC35500.031.02034811
CAGAGTA35500.031.01528410
TACGCGT450.004384970330.99868450
CGCAGAG36850.030.2513648
GCAGAGT37900.029.3227659
AGTACGG33800.028.32229213
GAGTACG34600.027.76660712
GTACGGG37100.025.71057514
Produced by FastQC (version 0.11.5)