FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636532
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGA14110.22166992390013385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12010.18867865244795234No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10400.1633853443346132No Hit
GTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10370.16291404045672486No Hit
CAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10010.1572583939220652No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9290.14594710085274581No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8230.12929436383402562No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8220.1291372625413962No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC7280.11436974103422923No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA6630.10415815701331589No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6560.10305844796490986No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCTCGGCAGCTACTTAGCCTGGTACC6470.10164453633124493No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGCAGCCTGAAGATT6470.10164453633124493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17200.043.4156651
GGTATCA5900.041.0333631
TCAACGC19100.039.0876124
ATCAACG19350.038.406433
TATCAAC19300.038.3353162
CAACGCA19950.037.2513475
AACGCAG20450.036.3377046
ACGCAGA20950.035.4704557
CGCAGAG21950.034.009788
AGAGTAC22400.033.17437711
ACCGATC959.726136E-932.34417327
GCAGAGT23650.031.5651059
TCGTATG901.9847539E-730.68073740
AGTACGG20050.030.6021813
GTGTAAC450.00489370630.3094641
CAGAGTA24900.029.84361510
GAGTACG20600.029.78513512
TCGAGTA500.00641048128.68030462
TGAGCGT656.787865E-427.66406463
TTACTCG500.008105963527.33436428
Produced by FastQC (version 0.11.5)