FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636532
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGA13740.21585717607284474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11620.18255170203540436No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11550.1814519929869983No Hit
CAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9460.1486178228274462No Hit
GTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9330.14657550602326355No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA8450.1327505922718732No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7370.11578365266789416No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7040.11059931001112276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6900.10839989191431068No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA6890.10824279062168123No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC6870.10792858803642237No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6620.10400105572068646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTCG350.005324259456.15347770
CCGCTAT902.654262E-643.67492770
GTATCAA18200.041.40871
GGTATCA7550.037.488831
TATCAAC21350.035.4282882
ATCAACG21500.034.8449973
TCAACGC21600.034.8440674
CAACGCA21750.034.6064835
AACGCAG23050.032.8024646
ACGCAGA23050.032.6547057
AGAGTAC23350.031.9484611
CGCAGAG24450.030.9242048
GCAGAGT25000.030.3801089
CAGAGTA26100.028.96922710
AGTACGG21950.028.55683713
TACGGGG11750.028.41509615
GAGTACG22500.028.01018512
GTACGGG24850.025.63551114
ACGGGGA4850.025.29239516
CTCGCTA951.1502361E-525.12330823
Produced by FastQC (version 0.11.5)