FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046102
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.039.9763371
GTATCAA25400.039.5011251
ATCAACG31000.032.5848053
TCAACGC31600.032.0764
TTAACGG1101.0750227E-931.31588235
CAACGCA32300.031.274845
TATCAAC32500.030.8716492
AACGCAG34050.029.6688866
AGAGTAC35050.028.82103511
CGCAGAG35650.028.5294428
ACGCAGA35500.028.4570587
TCGTATG1850.028.06939940
CAGAGTA36250.027.86828810
TAACGGC1306.35373E-926.51973236
GCAGAGT38600.026.2603659
CGTATGC2000.025.98805241
GTGGTAT4750.025.9528121
AGTACGG30500.025.82157713
GAGTACG30900.025.5993612
CTTATAC2300.023.8214241
Produced by FastQC (version 0.11.5)