FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046102
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22860.2185255357508159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.039.0564541
GTATCAA24750.038.334441
ACGTTAC451.0508869E-438.0212516
CGAATTA905.631591E-934.21257815
ATCAACG29850.031.7377343
CAACGCA30250.031.4326255
TCAACGC30250.031.4326254
TATCAAC30750.031.0401732
AACGCAG32450.029.30166
AGAGTAC33550.028.95118111
CGCAGAG33550.028.5447838
ACGCAGA33800.028.2324627
TGGTATC5050.027.775222
CAGAGTA34950.027.49790610
GTGGTAT5450.027.0244271
GCAGAGT37750.025.4583269
GAGTACG31750.024.99584412
AGTACGG32000.024.90746113
GTACGGG35450.022.09748314
TACGGGG14300.021.53239315
Produced by FastQC (version 0.11.5)