FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567839
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAATAGTTACCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24190.42600103198265704No Hit
CTATTAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATTGTGAGAGTGAATTCTGTC24130.4249443944498352No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18000.31699125984654103No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14850.2615177893733963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13090.23052308841062347No Hit
GATCTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11430.20128945000255355No Hit
GTCTCCACCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCA11050.1945974122946821No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10900.1919558184626276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10510.1850876744992859No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCACTTATTTAGCCTGGTATCAGCAGAAA10410.18332661194458288No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10270.18086112436799867No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10160.17892395555782536No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT10130.17839563679141446No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9760.17187970533901334No Hit
GGGTAACTATTAAGGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG9480.16694873018584494No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC9170.1614894362662656No Hit
TAGTTACCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT9160.1613133300107953No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9020.1588478424342111No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8960.15779120490138931No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8950.157615098645919No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8810.1551496110693348No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8800.1549735048138645No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8470.14916199838334457No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8330.14669651080676036No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8240.14511155450752766No Hit
GTAATACACGGCCGTGTCAGCGGCGGTCACAGACCTCAGTTTCAGGGAGA8160.14370270446376526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8090.14246996067547316No Hit
GGCCAAAAGTGTACATGGGGTAACTATTAAGGTGTTGACAGTAATAAGTT7930.13965226058794833No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7910.13930004807700774No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7770.13683456050042353No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7640.13454517917930964No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7540.13278411662460662No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCACCCTTC7430.13084694781443332No Hit
GTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAAATAAGTGCTAATG7290.1283814602378491No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA7100.1250354413839134No Hit
TATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGAT7040.1239788038510916No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6670.11746287239869048No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6460.11376464103381417No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6350.11182747222364085No Hit
GAATTCACTCTCACAATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACACCTTAATAGTTAC6290.11077083469081905No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6090.10724870958141305No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6090.10724870958141305No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6060.10672039081500215No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG5900.10390269072747733No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5900.10390269072747733No Hit
GTGTACATGGGGTAACTATTAAGGTGTTGACAGTAATAAGTTGCAAAATC5790.10196552191730404No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT5780.10178941566183373No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5700.10038056561807132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)