FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098529
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24650.2243909810300866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22160.20172430586720969No Hit
GATCTATGCTGCATCAACTTTGCAAAGTGGGGTCCCATCAAGGTTCCGTGGCAGTGGATCTGGCAAAGATTTCA21470.19544317901484623No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17980.16367342145723965No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17230.15684610966119236No Hit
GTCCTGATCTATGCTGCATCAACTTTGCAAAGTGGGGTCCCATCAAGGTT17210.15666404801329778No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17130.15593580142171942No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16380.14910848962567216No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15340.1396412839351533No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15220.13854891404778572No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTATCTGCTGATGCCAATT15130.13772963663226007No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG14870.13536283520963033No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14280.1299920165967398No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTATCTGCTGATGCCAATTTAAAT14270.12990098577279252No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13950.12698799940647904No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13480.12270955068095608No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13180.11997862596253718No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13120.1194324410188534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13000.11834007113148584No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12740.11597326970885613No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGAAGTGAAACCCTGCTGACA12680.11542708476517233No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12530.11406162240596288No Hit
GTTGGAGACAGCGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAACTATTTAAATTGGCATCAGCAGATA12220.11123966686359668No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGCGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAA12200.1110576052157021No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT12070.10987420450438723No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12010.10932801956070345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11790.10732534143386294No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCC11730.10677915649017913No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11400.10377513929991836No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATTTACATGACCCAGTC11390.10368410847597105No Hit
GGGTTTCACTTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT11150.10149936870123592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)