FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098529
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23550.21437759039588394No Hit
GATCTATGCTGCATCAACTTTGCAAAGTGGGGTCCCATCAAGGTTCCGTGGCAGTGGATCTGGCAAAGATTTCA19330.1759625826901247No Hit
GTCCTGATCTATGCTGCATCAACTTTGCAAAGTGGGGTCCCATCAAGGTT18550.16886217842223555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17140.1560268322456667No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16580.15092910610461807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16580.15092910610461807No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15990.14555828749172758No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15720.14310045524515055No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGTATCTGCTGATGCCAATT14480.13181263307568578No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG14130.12862655423753036No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTATCTGCTGATGCCAATTTAAAT14110.12844449258963578No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14050.127898307645952No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13930.12680593775858442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13480.12270955068095608No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13140.11961450266674799No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12750.11606430053280341No Hit
GTTGGAGACAGCGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAACTATTTAAATTGGCATCAGCAGATA12590.11460780734964665No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12580.11451677652569937No Hit
GGGTTTCACTTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT12540.11415265322991018No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12300.11196791345517505No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12100.11014729697622912No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGAAGTGAAACCCTGCTGACA11950.10878183461701967No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11880.10814461884938859No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11670.10623297154649536No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11640.10595987907465346No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11340.10322895435623455No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGCGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAA11220.10213658446886702No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11170.10168143034913052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.041.7934571
GGTATCA10650.038.0061721
GTATCAA24050.037.6539541
TTGTACG501.9398173E-434.2584463
TATCAAC27300.032.8900532
TCAACGC27600.032.269514
CAACGCA28050.031.9960655
ATCAACG27900.031.9254343
AACGCAG28650.031.2064236
ACGCAGA29700.029.987837
AGAGTAC30650.029.05967511
CGCAGAG30700.028.7826258
GCAGAGT31750.027.8307579
CCGTATT950.00793094227.45651470
CAGAGTA34500.025.8109110
ATACGAG801.003537E-425.6914965
AGTACGG31850.024.09385313
GAGTACG32150.023.86902612
ACGGGTC1653.3924152E-922.84832816
GTACGGG36300.020.952414
Produced by FastQC (version 0.11.5)