FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979674
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG114150.5766100883276741No Hit
GATTATTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTC102870.5196310099541641No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC88510.4470938144361143No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC79810.4031471848395241No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT76830.38809420136850814No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC75650.38213362402092466No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTG74450.37607201993863637No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC66560.3362169730975908No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG65920.33298411758703705No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG63180.3191434549324788No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC60380.3049997120738061No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC59270.2993927282976894No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT59250.2992917015629846No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG56630.2860571993166552No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC55970.28272331707139664No Hit
CTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC53900.2722670500294493No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT52950.26746828013097107No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG52890.26716519992685667No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG52220.26378080431424566No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT50890.2570625264563762No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA50690.2560522591093281No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG49270.24887936094528695No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCTCCGATGTTGGAG49080.2479196069655913No Hit
CCCCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTA48250.24372699747534193No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC46950.2371602597195296No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC45550.23008838829019324No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACGGCCCCA45500.2298358214534312No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT45480.22973479471872643No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCA44010.22230932971792325No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA43130.21786415339091186No Hit
CCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT42800.21619721226828256No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA42710.21574259196211093No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG42330.21382308400271963No Hit
GCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAA41210.20816558685925057No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACC40950.2068522393080881No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT40950.2068522393080881No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39860.20134628226667622No Hit
GTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG38960.19680007920496No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37980.19184976920442456No Hit
TTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCG37290.1883643468571088No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCA37220.18801075328564198No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGT37160.18770767308152755No Hit
CATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC36500.184373790836269No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG35830.18098939522365803No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA35810.18088836848895323No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC35450.17906988726426673No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA35140.17750397287634226No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC34620.17487727777401735No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG34520.17437214410049331No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG33940.17144236879405397No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC33810.17078569501847274No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT33670.1700785078755391No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC33250.1679569464467382No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC32880.16608795185469932No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTGG32700.16517871124235609No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG32310.16320868991561238No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCT32000.16164277552768788No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGC31620.1597232675682966No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC31380.15851094675183894No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC31130.15724811256802887No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG31070.15694503236391447No Hit
GTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCT30590.15452039073099916No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC30520.15416679715953233No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT29660.14982264756722571No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAATTCTGTAAAGTGGTATCAGCAAGTCCCAGGAACGGCCCC29440.14871135348547287No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC29390.14845878664871084No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGT29270.14785262624048204No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA28800.1454784979749191No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC28750.1452259311381571No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28640.14467028409728067No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCA28330.1431043697093562No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC28320.1430538563420038No Hit
CCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCC28190.14239718256642256No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA27990.14138691521937452No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG27740.14012408103556442No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCT27570.1392653537905736No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT27330.13805303297411592No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACCCCTGAGGACCGCTG26760.135173771035029No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAGCT26470.13370888338180933No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG26280.13274912940211367No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC26150.13209245562653243No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG25590.1292637070547979No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT25530.1289606268506835No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAG25270.12764727929952105No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG25150.12704111889129222No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC25020.12638444511571098No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC25020.12638444511571098No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG24920.12587931144218695No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT24570.12411134358485286No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC24200.12224234899281397No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGA23780.12012078756401307No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGG23620.11931257368637462No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA23440.11840333307403138No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23290.11764563256374534No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG23130.11683741868610689No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC23070.11653433848199249No Hit
TTCCTGGGACTTGCTGATACCACTTTACAGAATTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA23020.11628177164523049No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC22600.11416021021642957No Hit
GCTTGGTCCCTCCGCCGAAAACCACCGGACCACTGGGGCCGTCATCCCACGCTGCACAGTAATAATCAGCCTCAT22580.11405918348172477No Hit
ATCCCACGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC22410.11320045623673394No Hit
GTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC22300.11264480919585751No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC22120.11173556858351426No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21950.11087684133852342No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC21950.11087684133852342No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA21850.11037170766499937No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG21800.11011914082823737No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21730.10976554725677055No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC21320.10769449919532205No Hit
TCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG21280.10749244572591246No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT20990.10602755807269278No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC20760.10486575062358752No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG20390.10299675603154862No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGAT20290.1024916223580246No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAC19900.10052160103128091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA150.002413716468.5786138
TACGGTC150.002527677767.7853210
AGGGGGG5350.051.1672970
GTATCAA40300.039.7046431
TCGTATG3700.039.0237140
CGTATGC3850.037.57357441
CTTATAC4150.036.7594341
TTATACA4400.035.437712
TATCAAC44700.035.4135782
TATACAC4450.035.0395283
ATCAACG46900.033.969213
CGAGACG4300.033.90122223
TCAACGC47700.033.3275954
ACGAGAC4550.032.78111322
CAACGCA49150.032.482315
AACGCAG49450.032.2819826
AAGGGGG5500.032.13910369
ATCTCGT4200.031.7897137
ATGCCGT4700.030.92599344
AAAGGGG5150.030.4301168
Produced by FastQC (version 0.11.5)