FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979674
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTC100870.5095283364836837No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC92550.4675012148464848No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC77870.39334759157315796No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG71880.36309008452906893No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT71070.3589985017735243No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC62600.3162136796260394No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG62460.3155064924831058No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTG61910.31272825727872366No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC57570.290805455847781No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG56820.2870169532963508No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC56790.2868654131942936No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT56210.28393563788785425No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC55940.2825717769693394No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC55890.2823192101325774No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG55850.2821171566631678No Hit
CTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC53160.26852906084537154No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG52910.26726622666156147No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG52000.2626695102324928No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT50050.2528194035987743No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCTCCGATGTTGGAG49210.24857628074117255No Hit
CCCCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTA47410.2394838746177401No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC47250.23867566074010166No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA46890.23685717951541516No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT45730.2309976289025365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45230.22847196053491636No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC44880.22670399267758226No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG44680.2256937253305342No Hit
CCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT44250.22352165053438092No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACGGCCCCA43970.22210727624851365No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG43690.2206929019626464No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA43400.21922801430942668No Hit
GTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG42530.21483335134976767No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCA42440.21437873104359606No Hit
GCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAA40670.20543786502222083No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG40390.20402349073635356No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT39980.20195244267490506No Hit
CATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC39440.19922472083787535No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39160.19781034655200805No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA38810.19604237869467397No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT37280.1883138334897564No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCA36300.18336352348922094No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA35990.18179760910129647No Hit
TTACTGTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCG35400.17881732042750473No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC34280.17315982328403565No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAATTCTGTAAAGTGGTATCAGCAAGTCCCAGGAACGGCCCC33810.17078569501847274No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG33760.17053312818171074No Hit
GTTTGGGGGCCGTTCCTGGGACTTGCTGATACCACTTTACAGAATTACCT33550.16947234746731027No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGT33540.16942183409995787No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC32090.16209739583385951No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31630.15977378093564898No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC31260.1579047863436101No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT31220.1577027328742005No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA31060.15689451899656207No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG31010.15664195215980006No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC30840.1557832249148092No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT30690.15502552440452316No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG30470.15391423032277032No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG30310.1531060164451319No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC30300.15305550307777946No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30200.15255036940425545No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC29900.15103496838368338No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCT29760.15032778124074972No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGC29410.14855981338341565No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC29340.14820621981194884No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC28950.14623619848520514No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCT28840.1456805514443287No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC28050.14168999542348892No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGT27370.13825508644352555No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACCCCTGAGGACCGCTG27200.1373963591985347No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCACTGG27000.13638609185148665No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAG26970.13623455174942944No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC26890.13583044481061024No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA26690.13482017746356217No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT26660.13466863736150497No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA26480.13375939674916174No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG26430.13350682991239973No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACCGGACCA25820.13042551450390316No Hit
CCTGAGGACCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCC25530.1289606268506835No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAGCT25220.12739471246275902No Hit
GAATTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCG24900.12577828470748215No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCTTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG24020.12133310838047073No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC23750.11996924746195585No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC23740.11991873409460346No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC23660.11951462715578424No Hit
TTCCTGGGACTTGCTGATACCACTTTACAGAATTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGA23260.11749409246168813No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT23240.11739306572698333No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC23210.11724152562492612No Hit
GAACAAGAGATGGTGACCCGCTGCCCGGGGGTCCCAGACGCTGAGGGTGG23170.11703947215551652No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA23160.11698895878816411No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG23150.11693844542081172No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGA23000.11618074491052566No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG22920.11577663797170647No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC22770.1150189374614204No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC22730.1148168839920108No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC22530.11380661664496276No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA22500.11365507654290555No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAC22430.11330148297143874No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGG22360.1129478893999719No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG22090.11158402848145704No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22090.11158402848145704No Hit
GCTTGGTCCCTCCGCCGAAAACCACCGGACCACTGGGGCCGTCATCCCACGCTGCACAGTAATAATCAGCCTCAT22020.11123043490999024No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT21690.10956349378736095No Hit
GCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGTCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGT21660.10941195368530375No Hit
GTGCAGCGTGGGATGACGGCCCCAGTGGTCCGGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC21240.10729039225650283No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21050.1063306382768072No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC20340.1027441891947866No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20340.1027441891947866No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC20210.10208751541920537No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT20200.10203700205185298No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19800.10001646735775689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40700.039.6278081
TATCAAC45100.035.6495822
ATCAACG48600.033.1468473
TCAACGC49350.032.6430974
CAACGCA50600.031.83755
AACGCAG51550.031.448536
CGCAGAG54250.029.820218
CGGGTAT803.2005792E-629.62328517
AGAGTAC54050.029.55709511
CAGAGTA54850.029.43156210
GCAGAGT55750.028.8357529
ACGCAGA56800.028.5417587
CACAGTA40200.026.89138270
GATTGAC2350.025.9532741
ACGGGTA1850.025.62013816
GCCAGAG43450.025.21390370
GTATTAG1751.0913936E-1125.1705571
GCGGTAC1101.122411E-625.16960144
GTTCTAA700.001481961524.2024571
GCGTAGA1500.00148789124.18419570
Produced by FastQC (version 0.11.5)