FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978374
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29800.304586998428004No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29470.3012140551568214No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG26880.2747415609981459No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23920.24448728195965963No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20980.21443742372548738No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20660.21116669085646184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18300.18704503594739844No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17460.17845936216620636No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC17080.17457536688423855No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT16810.17181568602599823No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16730.17099800280874186No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16650.17018031959148547No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16370.16731842833108812No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT16070.16425211626637667No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15870.1622079082232357No Hit
GTAATATACAGCCGTGTCCGCGGCGGTCACAGAACTCATCTTCAGGGAGA15820.16169685621245045No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15670.16016370018009474No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15120.1545421280614571No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT15090.15423549685498594No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15060.1539288656485148No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14940.15270234082263018No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14460.14779624151909188No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGACGCAGACAGGGATGATGGAGACTGGGTCAT14100.14411666704143813No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13820.1412547757810408No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTGACAACTATTTAAATTGGTATCATCAGAAG13770.14074372377025554No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12750.1303182627502366No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA12450.12725195068552517No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12390.12663868827258287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12390.12663868827258287No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12380.12653647787042582No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12310.1258210050553265No Hit
CCATCATCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCA12230.12500332183807011No Hit
GTCTCCTACAGACGCAGACAGGGATGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG11940.12203922017551572No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG11820.12081269534963113No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCATCC11620.11876848730649014No Hit
CAGTAATATGTTCCAATGTCTTCAGGCTCAAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA11380.116315437654721No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11230.11478228162236526No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11100.11345354639432363No Hit
AGTCAGGACATTGACAACTATTTAAATTGGTATCATCAGAAGCCAGGGAA10990.1123292319705961No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC10930.11171596955765381No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10770.11008060312314104No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGA10400.10629881824333026No Hit
GCCTTGAGCCTGAAGACATTGGAACATATTACTGTCAACAGAATGATGAA10360.10588997663470207No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10320.10548113502607387No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10320.10548113502607387No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGATGATACCAATT10240.10466345180881749No Hit
GATCTACGATGCATCCAATTTAGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA10210.10435682060234634No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT10160.1038457685915611No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10060.10282366456999062No Hit
ATATTACTGTCAACAGAATGATGAATGGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG9980.10200598135273423No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9860.10077945652684965No Hit
GTAATATGTTCCAATGTCTTCAGGCTCAAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA9810.1002684045160644No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9800.10016619411390736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATCG208.6909124E-568.7131540
TCGTATG1500.048.099240
TATCTCG1500.047.74136736
CGTATGC1450.047.48229641
CTCGTAT1550.046.4557339
GTGGTAT3150.043.915751
GTATGCC1900.041.76999342
CTATCTC1850.038.6612835
AGACCGG2100.038.5956825
GCCTATC1850.038.57370433
TTATACA2100.038.5562252
CTTATAC2050.037.8547971
ACGAGAC2100.036.9591622
GTATCAA20850.036.89571
CCTATCT2050.036.50752334
AGCCTAT2050.036.43054232
ATGCCGT2000.036.41558544
GACCGGA2250.036.033726
ATCTCCG2150.034.52127510
CACGAGA2250.034.49345421
Produced by FastQC (version 0.11.5)