FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002458
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG43260.4315392764584651No Hit
GATTATTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTA37560.37467903892232896No Hit
GACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAA25160.250983083580559No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCC24830.2476911750916248No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21100.21048263368639883No Hit
GCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAATTGTCATCCCATG20180.20130519183846107No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT20150.20100592743037612No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19860.19811303815222187No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTC19690.19641720650640723No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19070.19023240873931874No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18880.1883370674881142No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGGGTCAC17880.17836158721861664No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17640.17596747195393722No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17400.1735733566892578No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17320.17277531826769799No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17250.17207703464883317No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17200.1715782606353583No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG16720.16679003010599946No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16670.16629125609252457No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15850.15811136227153658No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15510.1547196989799074No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15380.1534228865448727No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15300.1526248481233129No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC15250.152126074109838No Hit
CCCTCAACGTCTGCGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG15020.14983171364785358No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14890.14853490121281887No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14410.14374667068346006No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14060.1402552525891359No Hit
GTAATAGACAGCCGTGTCCTCACGTCTCGGGAGGTCCATTTGCAAAAATA13840.13806064692984643No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACAC13580.13546702205977706No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13260.13227486837353786No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13070.1303795271223333No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12910.1287834502792137No Hit
GTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACT12420.12389546494715989No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12170.12140159487978548No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12110.12080306606361564No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12030.12000502764205584No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11890.11860846040432615No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11690.11661336435042666No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11530.11501728750730703No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11510.11481777790191708No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG11380.1135209654668824No Hit
GTCAGGGACCCCGGAGGACCGCTGATTATTCCTATAGATGAGTAGTTTGG11270.11242366263723767No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG11230.11202464342645778No Hit
GTCTATTACTGTGCCAAAGATCTAGGAGGATACGCTTATGGTTTTGAAAGACTTGATTCTTACGGTCTGGACGT11220.11192488862376279No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11160.11132635980759295No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10990.10963052816177835No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTC10950.10923150895099845No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10850.10823396092404869No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10800.10773518691057382No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10790.10763543210787885No Hit
GCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGG10680.1065381292782341No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG10620.10593960046206424No Hit
ATTTAGTCCCTCCGCCGAACACCCAACCACTCAAATTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT10600.1057400908566743No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAACATC10600.1057400908566743No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG10590.10564033605397931No Hit
GTTTGGGGGCCGTTGGGGCCGCTCCTGGAAATTGCTGGTACCAGTCTACATAATTATTGCCGATGTTGGATCTG10380.10354548519738484No Hit
CCCCGGAGGACCGCTGATTATTCCTATAGATGAGTAGTTTGGGGGCCGTT10360.10334597559199489No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC10300.10274744677582504No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCGGAGGACCGCTG10240.10214891795965517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)