FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002458
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTA36720.36629963549595096No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCC28300.2823060916267814No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT22320.2226527196151859No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21750.21696669586157227No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20860.2080885184217194No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGGGTCAC20820.20768949921093952No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19440.19392333643903284No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG19320.19272627880669316No Hit
GACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAA18800.18753902906655442No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18760.1871400098557745No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18010.17965839965365132No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17920.17876060642939656No Hit
CCCTCAACGTCTGCGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG17070.17028144820032362No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTC16980.1693836549760688No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16780.1673885589221693No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16670.16629125609252457No Hit
GCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAATTGTCATCCCATG16010.15970743911465618No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15910.15870989108770642No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC15490.15452018937451745No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14660.14624054075083445No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACAC14570.14534274752657966No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14180.1414523102214756No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14070.14035500739183088No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14070.14035500739183088No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG13480.1344694740328273No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13140.13107781074119812No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13060.13027977231963833No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC12740.1270876186333991No Hit
GTAATAGACAGCCGTGTCCTCACGTCTCGGGAGGTCCATTTGCAAAAATA12640.12609007060644933No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12270.12239914290673524No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12270.12239914290673524No Hit
GTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACT12260.12229938810404027No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12020.11990527283936087No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11820.11791017678546134No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11790.1176109123773764No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11740.11711213836390152No Hit
CTCTTGTTCTGGAAGCAGATCCAACATCGGCAATAATTATGTAGACTGGT11670.11641385474503671No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG11570.11541630671808695No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11470.11441875869113718No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11450.11421924908574722No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11330.11302219145340753No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11220.11192488862376279No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11210.11182513382106782No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG11020.10992979256986327No Hit
GTCAGGGACCCCGGAGGACCGCTGATTATTCCTATAGATGAGTAGTTTGG10910.10883248974021854No Hit
GTCTATTACTGTGCCAAAGATCTAGGAGGATACGCTTATGGTTTTGAAAGACTTGATTCTTACGGTCTGGACGT10760.10733616769979391No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10690.10663788408092907No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCGGAGGACCGCTG10630.10603935526475922No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG10550.10524131684319941No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT10450.10424376881624967No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10430.1040442592108597No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10260.10234842756504511No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10080.10055284111653556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)