FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814077
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21100.041.8727071
GGTATCA8550.037.7956731
TATCAAC24950.035.271432
TCAACGC25050.034.9848024
ATCAACG25350.034.4415553
CAACGCA25500.034.3674245
AACGCAG26700.032.822826
TCTCGTA1305.456968E-1231.90996738
ACGCAGA28650.030.5888067
TCGTATG1251.200533E-1030.46978240
AGAGTAC28550.030.45519311
CGCAGAG29000.030.1011228
CAGAGTA29400.029.92537510
CTCGTAT1301.9099389E-1029.27793739
TAAGTCG605.5403524E-428.64303622
GCAGAGT31000.028.3808389
CGTATGC1352.8921932E-1028.2442241
AGTACGG23600.027.81431213
GAGTACG23600.027.66868812
TACCGCA500.0078802827.4939377
Produced by FastQC (version 0.11.5)