FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814077
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9310.11436264628530224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT251.0328525E-466.35551569
CGCGTTT556.810129E-445.28946770
GTATCAA19900.041.258811
TATCAAC23100.035.5127262
TCAACGC22900.035.2190064
GGTATCA8700.034.7484361
ATCAACG23550.034.6734733
CAACGCA23500.034.4658365
AACGCAG24600.032.785176
AGAGTAC25450.031.55921211
ACGCAGA25600.031.50457
CGCAGAG26000.030.8878178
GTGGTAT3350.030.7643871
CAGAGTA26150.030.7106410
GCAGAGT27650.029.0446039
CGACTAA500.006528781728.57415466
AGTACGG24200.027.80132313
GAGTACG24300.027.54565412
TACGGGA7250.025.09666315
GTACGGG27000.024.7910914
Produced by FastQC (version 0.11.5)