FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082542
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTA200.007007432652.39447456
GTATCAA23550.043.568741
TATCAAC26750.038.6106382
ACGGGTA655.5409873E-737.0761116
ATCAACG28700.035.9856033
TCAACGC28950.035.5559354
CAACGCA29150.035.3136145
AACGCAG29700.034.7739686
CTTACGC400.00262425834.424649
ACGCAGA31250.032.9390147
AGAGTAC32750.031.42890711
CGCAGAG33500.030.7252738
GTGGTAT4050.030.6053331
CTCGTAT802.7113092E-630.25853239
CAGAGTA34350.029.9649710
GCAGAGT35000.029.4084789
AGTACGG32250.028.81929613
GAGTACG33700.027.68144212
TCGTATG906.761111E-626.9064640
CTTATAC1608.0035534E-1125.823251
Produced by FastQC (version 0.11.5)