FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082542
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.038.039151
GTATCAA24300.037.3696941
TATCAAC26450.034.0515252
ATCAACG27200.032.97863
TCAACGC27450.032.676744
CAACGCA27500.032.618835
AACGCAG28550.031.539576
GGTATCA14850.030.8068371
AGAGTAC29950.030.07082611
CAGAGTA30350.029.6690210
ACGCAGA30550.029.4747877
CGCAGAG31800.028.3161898
GCAGAGT32450.027.9608159
AGTACGG30800.027.34616313
GAGTACG32500.025.91574712
GTATTAA809.757432E-525.7978291
GTACGGG35950.023.33307514
TACGGGA16100.022.2089815
TATTCTA2103.6038997E-521.67380370
ACGGGAT3100.021.07332816
Produced by FastQC (version 0.11.5)