FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793097
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC9990.12596189369017913No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9190.11587485515643105No Hit
GACTTACAGTGTCCCCCATTTTTTCGGCCCTGGGACCACAGTGGATATCAGGCGAACTGTGGCTGCACCAT8520.10742696038441703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23950.043.1840931
TCGTATG2100.041.1438640
CTCGTAT2000.039.6919239
TTGCGTG350.001398947439.1295439
CGTATGC2250.038.44252841
TCAACGC27250.038.0705344
ATCAACG27600.037.7141843
TATCAAC27550.037.6630942
TATACAC3150.036.958013
CAACGCA28250.036.84415
AACGCAG28350.036.834916
TTATACA2750.036.112732
GTGGTAT3050.035.9312671
CTTATAC2500.035.616871
ACGCAGA30600.034.0145727
CGCAGAG30850.033.9608968
AGAGTAC30700.033.7922511
GCAGAGT33150.031.5013489
AGTACGG27250.031.28568613
GAGTACG27500.031.00127412
Produced by FastQC (version 0.11.5)