FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578855
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20450.039.9545331
GGTATCA7900.038.1948851
TATCAAC23700.034.904692
CAACGCA23950.034.6789055
ATCAACG24300.034.0399063
TCAACGC24400.034.0393334
TATACCT601.5124913E-533.90045
TCGTATG1253.6379788E-1233.259840
AACGCAG24800.033.216926
CGTATGC1400.032.2278441
CTCGTAT1359.094947E-1230.73641239
ACGCAGA27350.030.1199157
CAGAGTA27650.029.7905410
AGAGTAC27750.029.6831911
CGCAGAG28400.028.8844648
AGTACGG20850.028.61369313
GCAGAGT28950.028.4527999
GAGTACG21350.027.94357912
TCTCGTA1503.45608E-1127.61653738
AATCTCG1503.6379788E-1127.5076536
Produced by FastQC (version 0.11.5)