FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578855
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11080.19141235715334584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTAG350.001229354240.16969749
GTATCAA22350.032.6498571
GCGATAT450.00435700431.0372245
GGTATCA11050.030.1012231
CAACGCA24400.029.4384675
GTGGTAT5550.029.354091
AACGCAG24900.029.1194786
TATCAAC25100.029.0349832
ATCAACG24800.028.961153
TCAACGC24850.028.902884
ACGCAGA25700.028.2130377
AGAGTAC26150.027.46602611
GTCTATA500.00837360727.1525331
CGCAGAG27000.026.7291418
CAGAGTA27450.026.6589510
GCAGAGT27200.026.6571719
TGGTATC5700.026.1658382
TACCGCG1058.983043E-725.8148658
TACGGGT4950.025.32813615
AGTACGG22000.025.1013213
Produced by FastQC (version 0.11.5)