FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849477
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCT31830.3747011396423917No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27080.3187843814488209No Hit
GCTTAGGACGCTGCCCTGGTTTCTGCTGGTACCAAGCTAAGTGGTGCTTATTGTTGGAACTGTATAAAATAGG22870.269224475765677No Hit
GTATAAAATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTC19360.22790493444790147No Hit
ATTTTATACAGTTCCAACAATAAGCACCACTTAGCTTGGTACCAGCAGAA18880.22225439888307746No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18750.2207240455009376No Hit
ATTTTATACTACTCGCCCCAATTTCGGCCCTGGGACCAGAGTGGAGATCAAACGAACTGTGGCTGCACCAT17300.20365471931553178No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16650.19600295240483262No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16390.19294224564055296No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCCGCCA15510.18258293043837562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15290.17999310163783128No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTCTGGTCCCAGGGCCGAAATTG14690.17292993218180128No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14500.17069326185405845No Hit
GTATAAAATAGGCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA13620.1603339466518811No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13440.1582149958150721No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAAACTGTTCATTTGCAGATACA13210.1555074475235939No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12850.15126954584997593No Hit
GTTTATTACTGTCAGCAATTTTATACTACTCGCCCCAATTTCGGCCCTGGGACCAGAGTGGAGATCAAACGAA12370.14561901028515192No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12340.14526585181235044No Hit
GTCCCTGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCAC12290.14467725435768125No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12170.14326462046647526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12100.14244058402993842No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12100.14244058402993842No Hit
ATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCACCATCGGCAGCC12090.14232286453900458No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11840.13937987726565876No Hit
GTCCCAGACCCGCCGCCACTGAATCGGTCAGGGACCCCGGATGCCCGGGT11830.13926215777472492No Hit
GATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATTTTATACTACT11420.13443565864643775No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11310.1331407442461656No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11200.1318458298458934No Hit
GAGTGAAATCTGTCCCAGACCCGCCGCCACTGAATCGGTCAGGGACCCCG11060.13019775697281974No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10710.1260775747901356No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10210.12019160024344391No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10200.12007388075251009No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10020.11795492991570108No Hit
GCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCTGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTT10000.11771949093383341No Hit
GAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9770.11501194264235523No Hit
GTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGACGCTGCCCTGGTTTCTGCTGGTA9710.11430562569675223No Hit
GTCTGGGACAGATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAAGATG9690.11407018671488456No Hit
TCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCCGCCACTGAATCGGTCAGGGACC9680.11395246722395073No Hit
GTAAATGAGCAGCTTAGGACGCTGCCCTGGTTTCTGCTGGTACCAAGCTA9470.11148035791434024No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9380.11042088249593573No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9240.10877280962286207No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATTTTATACTACTCGCC9190.1081842121681929No Hit
CCACTGAATCGGTCAGGGACCCCGGATGCCCGGGTAGATGCCCAGTAAAT9120.10736017573165606No Hit
CCACTTAGCTTGGTACCAGCAGAAACCAGGGCAGCGTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGCATC9080.10688929776792072No Hit
GTTCCAACAATAAGCACCACTTAGCTTGGTACCAGCAGAAACCAGGGCAGCGTCCTAAGCTGCTCATTTACTGGG8930.10512350540391323No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8870.10441718845831023No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8520.10029700627562606No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8500.1000615672937584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)