FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474211
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54620.37050327259802024No Hit
CATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA46390.314676799996744No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35520.2409424431102468No Hit
GCATAGATGAGGCGCCTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGAC35260.23917878783973257No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34400.23334515886803178No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC32240.21869325354376No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31310.21238479430692075No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31280.21218129562186144No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31120.21109596930154506No Hit
CTGTAAGTCTGCTGACAATAGTAAGTCGCGAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC29540.20037837188842034No Hit
GACTTACAGTTTCCCGCTCGCTTTCGGCGGAGGGACCATGGTGGAGATCAAAGGAACTGTGGCTGCACCAT28440.1929167534362449No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28310.19203492580098777No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCG28150.19094959948067136No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27980.18979644026533515No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27510.18660829419940567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26950.18280965207829816No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT26710.1811816625978235No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT26420.17921450864225No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT25950.17602636257632048No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25840.17528020073110295No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24240.16442693752793866No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24200.16415560594785955No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAA23960.16252761646738495No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23730.16096745988193006No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22230.15079252562896356No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC21630.14672255192777697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21410.14523022823734186No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21360.144891063762243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21190.1437379045469068No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20960.14217774796145194No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT20840.1413637532212146No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20690.14034625979591794No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20550.13939659926564107No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTC20550.13939659926564107No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20520.13919310058058176No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20450.13871827031544331No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20450.13871827031544331No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19720.1337664689789996No Hit
GTAGGTGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATGTTAGCAGATGGGTAGTCTGGTATCAGCAGAAA19640.1332238058188414No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19110.12962866238279325No Hit
CCCCTAGGCGCCTCATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA19020.12901816632761523No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT18520.1256265215766264No Hit
GCCTTAGAGTCGAGGACACGGCCGTATATTATTGTGCCAGCCTCTATGAC18130.12298103867085512No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18070.12257404130073646No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17710.1201320570800245No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17640.11965722681488607No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17390.11796140443939165No Hit
CCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAACTGTAAGTCTGCTGA17160.1164012478539368No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17150.11633341495891701No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17000.11531592153362034No Hit
GTCTCCATCTTTCGTGTCTGCATCTGTAGGTGACAGAGTCACCATCACTT16920.11477325837346214No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC16510.1119921096776513No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTCCCGCTCGCTTT16280.11043195309219643No Hit
GGATGTTAGCAGATGGGTAGTCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAGGCGCCTCATCTATGCTGCATC16220.11002495572207778No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC16210.10995712282705801No Hit
GTCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAGGCGCCTCATCTATGCTGCATCCAGTTTGCAAAGTGGGGTC16100.10921096098184045No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG16040.1088039636117218No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC15990.10846479913662291No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAACT15800.10717597413124715No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.1067689767611285No Hit
CAATAGTAAGTCGCGAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG15190.10303816753504076No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15030.10195284121472434No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATGAGGCGCCTAGGGGCTTCCCCTGGTTTCTG14990.10168150963464524No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA14860.10079968199938816No Hit
GCTAACATCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCACCTACAG14860.10079968199938816No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14750.1000535201541706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)