FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449470
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG200.007532945451.440048
TACGATA200.007532945451.440047
CTCGTAT451.8202554E-646.14024439
TCGTATG551.4223042E-744.0825540
GGTATCA6300.041.9141041
CGTATGC602.7968963E-740.44536241
GGACCGA350.00138352239.21422225
GTATCAA14750.038.1295661
CAACGTT601.8025452E-434.68149669
TATCAAC17650.032.447542
ACGTGCG450.004111728631.40533460
CAACGCA18200.031.2785575
TCAACGC18200.031.2785574
ATCAACG18500.030.771343
AACGCAG19050.029.702916
TATACAC1302.1100277E-1029.0174583
AGAGTAC19750.028.47651111
CGCAGAG20150.028.2516028
ACGAGAC854.6197856E-628.25101922
ACGCAGA20250.028.112097
Produced by FastQC (version 0.11.5)