FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324895
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC3420.10526477785130581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG150.002153638870.5649457
TTACCGC200.006717332652.9489358
CGATAGT200.0073144151.8211337
CCGATAG200.007341430551.77283536
GCCGATA200.007354968751.74872235
TAGTACT200.007611106651.302684
GGTATCA5350.040.9268881
GTATCAA11650.034.652821
TTAACGG1207.6397555E-1131.6242235
TTTAACG1207.8216544E-1131.58988634
TGTACTA450.004664152430.6041433
TAACGGC1251.2369128E-1030.37339636
ATCAACG13750.030.1022013
TCAACGC13950.029.6660654
CAACGCA14000.029.5601145
TATCAAC14250.029.0459842
CGCGGTA1452.0008883E-1128.76988443
CATAACA854.485879E-628.35383233
AACGCAG14800.027.962276
CGGTACC1503.092282E-1127.9025645
Produced by FastQC (version 0.11.5)