FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324895
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3590.11049723756906078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG200.00759117151.3367234
GTCCGAT306.548867E-445.64669411
TCGTGCA405.2312615E-542.787199
GTATCAA11750.036.1567151
GGTATCA5600.035.4850271
TCCGATT400.002695567734.2297512
CGTGCAC400.002695567734.2297510
CAACGCA13400.030.3980625
AACGCAG13300.030.3692536
ATCAACG13450.030.2803963
TCAACGC13750.029.3708274
TATCAAC14400.029.2514272
ACGCAGA13900.029.0583527
TTAGCCT605.673777E-428.52044
TGCCGAG950.007615822427.67443770
CGCTCAA500.007808320727.54071432
GTTCATA500.00799299727.4091241
CTTGTAT756.468058E-527.3964582
CCTATAG500.008011050527.3964562
GGGTCGA500.00802913627.38386
Produced by FastQC (version 0.11.5)