FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863861
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT38490.44555779228371234No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA32430.3754076176607116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31630.366146868535563No Hit
GTTATTATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACT27300.3160230638956962No Hit
GTAGTACACGGCCGTGTCCGCGGCACTCAGAGAGCTCAGATTCAGGGAGA25480.2949548596359831No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23440.2713399493668541No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23020.2664780560761511No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCAGGGAAAGCAGTGGCCCTGGT22880.26485742497925013No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20460.23684365887567557No Hit
CTCCTGCAGGGCCAGTCAGTTTATTAGCGCCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT19470.2253834818333042No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT18370.21264995178622487No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18270.21149235814558132No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17970.20801957722365055No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17090.19783275318598711No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCACTTTATTACTGTCAGCAGTATAATAACTGG17000.1967909189094079No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGC16830.1948230097203138No Hit
CATCTATGGTGCATCCACCAGGGCCACTGCTTTCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA16780.194244212899992No Hit
GTGTACTACTGTGCGACCCACCTTAACGAACATAGAAGACTTGATGCTTT16130.18671985423580878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16020.18544650123110085No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15790.18278403585762062No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15710.18185796094510576No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15520.17965853302788296No Hit
GGTTTGTGCTGGTACCAGGCTAAGTTGGCGCTAATAAACTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC15370.1779221425669176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15320.17734334574659583No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15240.17641727083408096No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTTTATTAGCGCCAACTTAGCCTGGTA15100.17479663973717993No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15000.17363904609653638No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14920.1727129711840215No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTTACACTTTTGGCCAGGGGA14680.16993474644647694No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14550.16842987471364027No Hit
CAGTAATAAAGTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14430.16704076234486798No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTTTATTAGCGC14280.16530437188390262No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCAGGGAAAGCA14250.16495709379170956No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14070.1628734252385511No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGCTTTCCCTGCCAGGTT14050.1626419065104224No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14040.16252614714635805No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13980.1618315909619719No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13850.16032671922913524No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13670.15824305067597683No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC13550.1568539383072045No Hit
GTTTATTAGCGCCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC13170.15245508247275893No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13170.15245508247275893No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12610.14597255808515489No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12320.14261553652728853No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCACTTTATTACTGTCAGC12140.14053186797413011No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12060.13960579306161522No Hit
GGCCAAAAGTGTAAGGCCAGTTATTATACTGCTGACAGTAATAAAGTGCA11970.13856395878503602No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11770.13624877150374887No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11460.13266023121775378No Hit
GTGTAAGGCCAGTTATTATACTGCTGACAGTAATAAAGTGCAAAATCTTC11460.13266023121775378No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11430.1323129531255607No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11290.1306923220286597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11090.12837713474737256No Hit
CCTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCACTAGACACATCT11020.12756681919892204No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCACCAGCC10980.12710378174266462No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10560.1222418884519616No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10460.12108429481131802No Hit
GTAATAAAGTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA10390.12027397926286751No Hit
AGATAGCACTGCAGAGATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCT10380.12015821989880317No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10250.11865334816596651No Hit
CACCAGGGCCACTGCTTTCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCACCAG10220.11830607007377344No Hit
CTGCTACTCTGGCTCCCAGATAGCACTGCAGAGATAGTGATGACGCAGTC10040.116222401520615No Hit
CTATTACACTGGGAGCACCTCCTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCACTAGACACATCTAAGAA10040.116222401520615No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9970.1154120859721645No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9910.11471752978777836No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9750.11286537996274863No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9720.11251810187055555No Hit
AACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCA9530.11031867395333278No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG9460.10950835840488227No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT9440.10927683967675356No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9400.10881380222049612No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9190.10638285557514461No Hit
GCAGAGATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG9180.10626709621108027No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9150.10591981811888718No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9100.10534102129856539No Hit
CTTTATTACTGTCAGCAGTATAATAACTGGCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA9010.10429918702198618No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8910.1031415933813426No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGG8880.10279431528914953No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8870.10267855592508517No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACTGCAGAGATAGTGATGACGCAGTCTC8730.10105792482818417No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACTGCAGAGATAGTGATGACGCAGTCTCCAGCCACC8700.1007106467359911No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC8640.10001609055160494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAA150.002402224868.6579228
GTGGTAT1600.049.3050271
GTATCAA15350.045.3597831
TGGTATC1800.043.8216172
CTTATAC651.1979864E-842.2143361
GCACTAT350.001040288241.55919667
GGTATCA4850.040.3103371
CGTATGC702.165325E-839.4755441
TATCAAC18100.038.653132
ATCAACG19000.036.822193
TCAACGC19000.036.820064
CAACGCA19250.036.341885
AACGCAG19500.036.2276846
AGAGTAC20400.034.7995211
TACGCAT400.002551473734.62183844
TCGTATG807.0565875E-834.51896740
CTACCCG400.002672892834.29517710
CGCAGAG20650.034.212148
GCAGAGT21300.033.1681069
CTAGTGA6750.032.9339669
Produced by FastQC (version 0.11.5)