FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863861
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT34490.39925404665796926No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA32290.37378698656381065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31110.3601273816042164No Hit
GTTATTATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACT25610.29645973136881976No Hit
GTAGTACACGGCCGTGTCCGCGGCACTCAGAGAGCTCAGATTCAGGGAGA23130.2677514090808591No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCAGGGAAAGCAGTGGCCCTGGT21810.2524711730243639No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21350.24714624227740342No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21030.24344194262734398No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19330.2237628507364032No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19230.22260525709575962No Hit
CTCCTGCAGGGCCAGTCAGTTTATTAGCGCCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT19110.22121614472698733No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17570.20338920266107624No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17430.20176857156417524No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16960.19632788145315044No Hit
GTCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT16300.18868776342490284No Hit
CATCTATGGTGCATCCACCAGGGCCACTGCTTTCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA15550.18000581112007605No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15460.17896397684349682No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15450.17884821747943244No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCACTTTATTACTGTCAGCAGTATAATAACTGG15250.17653303019814529No Hit
GGTTTGTGCTGGTACCAGGCTAAGTTGGCGCTAATAAACTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC15230.1763015114700166No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15070.17444936164498687No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15050.17421784291685816No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTTTATTAGCGCCAACTTAGCCTGGTA15010.1737548054606007No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14990.173523286732472No Hit
GTGTACTACTGTGCGACCCACCTTAACGAACATAGAAGACTTGATGCTTT14710.17028202453867No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14610.16912443089802642No Hit
CAGTAATAAAGTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14480.16761955916518978No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14390.16657772488861056No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGC14280.16530437188390262No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14210.1644940563354521No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14170.1640310188791947No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGTTTATTAGCGC14160.16391525951513033No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTTACACTTTTGGCCAGGGGA13860.1604424785931996No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCAGGGAAAGCA13860.1604424785931996No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13740.1590533662244273No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13010.15060293264772923No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12960.15002413582740742No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12900.14932957964302127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12890.1492138202789569No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12770.14782470791018462No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGCTTTCCCTGCCAGGTT12570.1455095206288975No Hit
GTTTATTAGCGCCAACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC12560.1453937612648331No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12500.14469920508044695No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12290.14226825843509544No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11860.1372906057803281No Hit
GGCCAAAAGTGTAAGGCCAGTTATTATACTGCTGACAGTAATAAAGTGCA11790.13648029023187758No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11410.132081434397432No Hit
CTGCTACTCTGGCTCCCAGATAGCACTGCAGAGATAGTGATGACGCAGTC11300.13080808139272407No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11130.12884017220362998No Hit
GTGTAAGGCCAGTTATTATACTGCTGACAGTAATAAAGTGCAAAATCTTC10840.12548315064576362No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10760.12455707573324876No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCACTTTATTACTGTCAGC10490.1214315729035111No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10310.11934790435035267No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10270.11888486689409522No Hit
AGATAGCACTGCAGAGATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCT10270.11888486689409522No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10250.11865334816596651No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10000.11575936406435756No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9910.11471752978777836No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9690.11217082377836249No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9640.11159202695804071No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9590.11101323013771891No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9420.10904532094862483No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCACCAGCC9360.10835076476423869No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG9350.10823500540017433No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9300.10765620857985254No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACTGCAGAGATAGTGATGACGCAGTCTCCAGCCACC9280.10742468985172383No Hit
GTAATAAAGTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA9210.10661437430327333No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9050.10476222447824361No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9000.10418342765792182No Hit
CTATTACACTGGGAGCACCTCCTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCACTAGACACATCTAAGAA8970.10383614956572873No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.10360463083760003No Hit
AACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCA8950.10360463083760003No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8870.10267855592508517No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCACTGCAGAGATAGTGATGACGCAGTCTC8790.10175248101257031No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8730.10105792482818417No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8700.1007106467359911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACC150.002415330468.563771
GGTATCA6350.048.0486261
GTGGTAT1450.047.285361
TGGTATC1500.043.3784562
GTATCAA16250.040.5053631
TACGGGT1001.0913936E-1137.6620415
TATCAAC17700.036.3744432
TCGCTAT807.52425E-834.2679824
AATAGCG751.387576E-632.96925762
TGTTAGA7900.032.7307170
TAATAGC651.9966998E-532.5544561
ATCAACG19900.032.5120543
CAACGCA20250.031.950125
TCAACGC20400.031.717034
AACGCAG20400.031.7151936
ACGGGTA553.4050853E-431.12565416
GAGCATT900.0043403531.05976970
ACTTACT450.0046791230.58982735
CTCGCTA902.1189408E-730.45513223
CGCAGAG21350.030.1436428
Produced by FastQC (version 0.11.5)