FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796292
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38250.038.886531
GGTATCA17700.036.292021
TCAACGC44650.033.0707934
ATCAACG44800.032.8843353
TATCAAC45100.032.8224452
CAACGCA45150.032.552455
AACGCAG46100.031.9543386
TCGTATG1700.030.51986940
ACGCAGA48550.030.3418147
CGCAGAG51350.028.5536048
AGAGTAC51500.028.46964511
CAGAGTA54050.027.25354410
GCAGAGT57700.025.4700249
AGTACGG45800.025.19110913
GAGTACG46700.024.4843612
CGTATGC2200.023.60537741
CTCGTAT2200.023.56899539
GTGGTAT7850.022.7549781
TACGGGG23150.022.103315
GTACGGG52000.021.72652214
Produced by FastQC (version 0.11.5)