FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823669
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT8810.10696044163371451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.039.044521
GTATCAA25050.038.2338871
TCGTATG1450.038.2320240
TATCAAC28750.033.7793582
TCAACGC28850.033.6622774
CAACGCA28950.033.5500685
ATCAACG29300.033.0289763
CTCGTAT1600.032.44023539
AACGCAG29850.032.4243356
TAACGGC1400.032.0207436
ACGCAGA32350.029.9185927
TTAACGG1501.8189894E-1229.84751735
ACGAGAC1850.029.49233422
AGAGTAC33350.029.22586311
TACGGGT5650.028.95275515
CGCGGTA1700.028.655543
CGTATGC1950.028.4834441
CAGAGTA34150.028.44142210
AGTACGG25800.028.39639313
CGCAGAG34150.028.3416258
Produced by FastQC (version 0.11.5)