FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823669
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22590.27426065567600577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.038.1938971
GTATCAA25850.037.369041
TCAACGC28850.032.7275244
TATCAAC29300.032.585762
ATCAACG29100.032.333153
CAACGCA29400.032.2312135
AACGCAG30050.031.8743296
ACGCAGA31000.030.7875797
CGCAGAG32150.029.3682448
AGAGTAC32600.029.17020211
CAGAGTA33250.028.70246910
GCAGAGT34050.027.9280059
AGTACGG28550.027.8198913
GAGTACG30050.026.65646612
TACGGGG12150.026.37926115
GTGGTAT4050.026.1271951
TACGGGT7850.025.19239215
GTACGGG31800.024.97666214
ACGGGGG4400.024.80355316
CGGGATT1401.5663318E-824.36211217
Produced by FastQC (version 0.11.5)