FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571322
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT37630.2394798774535073No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32300.20555939520989333No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC31810.2024410019079476No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTGCCACTGCTGTCTG31730.2019318764708952No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTG31120.1980497950133709No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29360.18684903539821882No Hit
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATCGGAGGGTGTTCG29030.1847488929703778No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC28230.17965763859985412No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC27960.1779393402498024No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGG27420.1745027435496989No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27140.17272080452001565No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26830.1707479434514377No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26700.1699206146162276No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26620.16941148917917526No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCAC26050.16578397044017712No Hit
CCCCTATACTATTGATATATAAGGACACTGAGAGGCCCTCAGGGATCCCT25240.16062907539002189No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT24930.15865621432144397No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24030.15292855315460485No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23960.15248306839718404No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23490.14949195645450136No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATG23350.14860098693965973No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23330.14847370558039663No Hit
GGCCAGGCCAGGCCCCTATACTATTGATATATAAGGACACTGAGAGGCCC23280.1481555021822389No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23210.1477100174248181No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23060.1467554072303449No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22690.1444007020839777No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22500.14319152917097833No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22010.14007313586903258No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21510.13689110188745526No Hit
GGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT21130.13447275606145653No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC20960.13339086450772025No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20270.12899965761314358No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20220.12868145421498584No Hit
TCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCA20200.12855417285572276No Hit
ATAATACACAGCCGTGTCCTCAATTCTCAGGCTGTTCATTTGCAGATAGA19820.126135827029724No Hit
GGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATC19560.12448116935930383No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19210.12225374557219972No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19190.12212646421293663No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19110.12161733877588425No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18670.11881714887209624No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18440.11735341324057068No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18040.11480778605530884No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17990.1144895826571511No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGT17930.11410773857936182No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17900.1139168165404672No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17850.11359861314230947No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17810.11334405042378327No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17530.11156211139410001No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC17330.11028929780146908No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG16990.1081255146939965No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16880.10742546721804952No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCG16830.10710726381989179No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16780.10678906042173406No Hit
CCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG16730.10647085702357632No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16700.10627993498468168No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16660.10602537226615551No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATACGTCC16550.10532532479020851No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16440.1046252773142615No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC16060.10220693148826276No Hit
GTGTATTATTGTGCGAAAGAGGCGGGAGCGCCGGCCGCGTCGTTAGATCTCTGGGGCCGTGGCACCCTGGTCA15940.1014432433326842No Hit
GTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGA15850.10087047721600029No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC15840.10080683653636874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)