FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144433
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGA38930.3401684502281916No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34860.3046049877974508No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27590.24108008070371964No Hit
GTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27400.2394198699268546No Hit
CAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26070.22779839448879924No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23850.2084001422538497No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC21240.18559408895059826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20450.1786911072994225No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19930.17414737254168658No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19090.16680749331765163No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18400.16077830681219435No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGCAGCCTGAAGATT17820.15571029496702735No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17800.15553553593788366No Hit
GTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16970.14828303622842054No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGGAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCTCGG16960.1481956567138487No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCTCGGCAGCTACTTAGCCTGGTACC16500.14417619904354384No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16500.14417619904354384No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16290.1423412292375351No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT16270.1421664702083914No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16070.1404188799169545No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15420.1347392114697846No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15270.13342851875120693No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGGAGGAGCCACCCTCTCCTGC14550.1271371937020341No Hit
GTCTTTGTCTCCAGGGGGAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14470.12643815758545937No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14380.12565174195431275No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14360.12547698292516904No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14230.12434104923573508No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14200.12407891069201953No Hit
GGCCAAAAGTGTACGGTGAACTACCATACTGCTGACAGTAATACACTGCA13430.1173506880699875No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13380.11691379049712829No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13230.1156030977785506No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12960.11324385088511078No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12500.10922439321480594No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12450.1087874956419467No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12260.10712728486508166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12100.10572921263193213No Hit
CCATAGATGAGGAGCTTGGGAGTCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCGAGAACACTCTGA11810.10319520670934865No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11690.1021466525344865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.041.129341
GTATCAA26600.040.393971
TATCAAC30500.035.2273032
TCAACGC31950.033.5196654
ATCAACG32250.033.2093053
CAACGCA32350.033.10525
AACGCAG33100.032.1462756
CGTATGC1001.39971235E-831.16247641
ACGCAGA35550.029.930857
CGCAGAG36100.029.5699188
AGAGTAC36300.029.31244511
TCGTATG1103.5732228E-828.2995940
GCAGAGT38600.027.7436899
CGCATAA1156.9471105E-427.55468270
TGTTAGA7600.026.68453270
CAGAGTA41500.025.72226710
AGTACGG33400.025.69380613
GAGTACG33950.025.2753512
AACTGTG23400.024.9169770
TAGGACG750.001615515423.8452666
Produced by FastQC (version 0.11.5)