FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144433
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGA36320.3173623969249401No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33020.28852715711623134No Hit
GTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27980.2444878817720216No Hit
CAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24120.2107593891472895No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23880.20866228079756527No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20350.17781731215370408No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC19760.17266192079396522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18630.16278803564734676No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18580.16235113807448753No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18420.16095306584133803No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGCAGCCTGAAGATT17740.15501125885045258No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17330.15142869875300696No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT16830.1470597230244147No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16490.14408881952897198No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCTCGGCAGCTACTTAGCCTGGTACC15730.1374479764215118No Hit
GTAATACACTGCAAAATCTTCAGGCTGCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15220.1329916211783477No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14970.13080713331405158No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14770.1290595430226147No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGGAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCTCGG14470.12643815758545937No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14270.12469056729402246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14160.12372939263373217No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13850.12102062768200497No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13810.1206711096237176No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13400.11708854952627197No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGGAGGAGCCACCCTCTCCTGC13360.11673903146798459No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13290.11612737486598167No Hit
GTCTTTGTCTCCAGGGGGAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13070.1142050255454011No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12780.11167101962281757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12480.10904963418566224No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12420.10852535709823118No Hit
GGCCAAAAGTGTACGGTGAACTACCATACTGCTGACAGTAATACACTGCA12400.10835059806908749No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12390.10826321855451564No Hit
GACTTCACTCTCACCATCAGCAGACTGCAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGTTCA12120.10590397166107583No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12040.10520493554450107No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12020.10503017651535737No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11910.1040690018550671No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11720.10240879107820204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11630.10162237544705545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.042.1502081
GGTATCA11300.041.2866061
TATCAAC34400.036.0693242
ATCAACG35700.034.841233
TCAACGC36000.034.5523954
CAACGCA36300.034.2668345
AACGCAG36700.034.0800976
ACGCAGA38200.032.8301437
CGCAGAG39150.031.9445768
AGAGTAC39150.031.77092611
GCAGAGT41450.030.17079
CAGAGTA44500.028.02582710
AGTACGG39750.027.32726113
GAGTACG40050.027.29368612
TACGGGG20300.025.65904615
AACTGTG23700.023.86645770
GTATTAG2300.023.8638691
GTACGGG45550.023.69715314
TACGGGA15300.022.17362815
GTGGTAT8550.020.8634411
Produced by FastQC (version 0.11.5)